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KMID : 0811720050090000064
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Korean Journal of Physiology & Pharmacology
2005 Volume.9 No. 0 p.64 ~ p.0
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COX-independent Prostaglandins from Arachidonic Acid in Primary Cortical Neurons
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Park Jee-Young
Kwon Kyoung-Ja
Jang You-Na
Koh Ki-Young
Seok Sun-Mi
Jung Yi-Sook
Lee Soo-Hwan
Moon Chang-Hyun
Baik Eun-Joo
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Abstract
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Cyclooxygenase (COX) isoforms produces prostaglandins from arachidonic acid in many kinds of cells. In pathologic conditions, the expression of COX-2 in the brain is induced and a lot of prostaglandins are released in the brain; therefore the regulation of COX activity in the nervous system has been thought to be harmful to the neurons or helpful. In the present study, the various types of cells such as astrocytes, microglial cells, neurons and brain endothelial cells are investigated with the expression and activity of the COX isoforms. COX-1 expressed constituitively in all kinds of brain cells, COX-2 was induced by cytokines and lipopolysaccharides. However, COX-3 was constitutively expressed in endothelial cells, astrocytes and microglial cells, but not neurons. In according to AA concentration (100 nM-10μM), the cells produced PGE2 concentration- and time-dependently. Especially, primary neuron cultures have little release of PGE2 in basal conditions, however, neurons responded well to exposure of AA. Unlike other cells, AA-induced PGE2 release in neurons was not inhibited by COX inhibitors in all kinds of concentration of AA (100 nM-50μM), and all time points (10 minutes-24 hr). In neurons, PGE2, PGD2 and also PGF2a released from AA did not affected by all kinds of COX inhibitors. Recently, it is reported that the COX-independent prostaglandins released from neurons and it is called isoprostane, prostaglandin (PG) like compounds, might be produced non-enzymatically from AA according to oxidation. Then we examined that isoprostane was released from AA in pure cortical neuron and cell free system. In conclusions, cortical neurons have little conventional COX activity from other cells and produce COX-independent prostaglandins from exogenous AA. This study was supported by the Korea Science and Engineering Foundation through Chronic Inflammatory Disease Research Center (R13-2003-019).
Source: Korean Journal of Physiology & Pharmacology.2005 Oct;9(Suppl I):S87-S87
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KEYWORD
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Neuron, Arachidonic acid, Prostaglandin, COX, Isoprostane
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